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. 2019 Oct 11;9:14692. doi: 10.1038/s41598-019-51198-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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A two-channel MPA detection system with internal control and Candida-specific melt curve profiles. (a) The fluorescence negative derivative (−dF/dT) melt peaks obtained from dissociation between THO and PCO for the C. krusei-specific, pan-Candida and C. glabrata-specific probes in the FAM channel; (b) Melt peaks for the internal control (IC) and C. auris-specific MPA probes in the HEX channel. Their specific melting temperatures are indicated.