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. 2019 Oct 11;9:14692. doi: 10.1038/s41598-019-51198-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Amplification profiles and melt curve analyses of genomic DNA in MPA-Candida assays. Amplification reactions containing a range of 1 to 10⁵ GEs per reaction were set up. Post-amplification melt curve analysis was then carried out. Amplification plots of C. albicans (a), C. krusei (b) and C. glabrata (c) and their corresponding melt curve profiles are shown demonstrating changes in the pan-Candida peaks proportional to the number of GEs present in the reaction. Similar results can be seen for C. krusei and C. glabrata at their corresponding species-specific peaks in the FAM channel. (d) Amplification plots of C. auris and their corresponding melt curve profiles showing proportional changes to their pan-Candida (i) and C. auris-specific (ii) peaks in the FAM and HEX channels respectively. The horizontal dotted line is fixed as the threshold value of fluorescence.