Table 4.
Description of the MPA-Candida and pan Fungal assays.
| Reaction Type | Probe target | Detection channel | Primer set |
|---|---|---|---|
| MPA | C. krusei | FAM | Forward: LR043 & Reverse: GSCand (this study) |
| Pan-Candida | |||
| C. glabrata | |||
| C. auris | HEX | ||
| Internal Control | Forward: M13 (−20) & Reverse: M13 (−29) | ||
| qPCR | Pan-Fungal | FAM | Forwards: ITS344 & Reverse: LR145 |
| Internal Control | HEX | Forward: M13 (−20) & Reverse: M13 (−29) |
FAM: 6-carboxy-fluorescein; 465 nm/510 nm; HEX: Hexachloro-fluorescein; 538 nm/580 nm. For simplicity and flexibility, the MPA-Candida assay was engineered as a two-tube system, run simultaneously under the same experimental conditions.