Fig. 4.

Analysis of Tom⁺ and Fos⁺ learning-associated neurons in the auditory cortex of EphB2 mutant mice. Neurons activated exclusively following encoding (Tom⁺ single-positive), exclusively following sound-cued retrieval (Fos⁺ single-positive), and following both encoding and retrieval (Tom⁺/Fos⁺ dual-positive) were imaged and counted in the auditory cortex from EphB2^−/− mutants and WT littermates subjected to FC and WT home cage controls not subjected to FC (WT-h). Scatter plots show the number of Tom⁺ single-positive neurons, Fos⁺ single-positive neurons, and Tom⁺/Fos⁺ dual-positive neurons. Representative confocal images of Tom⁺ and Fos⁺ labeled neurons in the auditory cortex are shown with the box indicating quantification area. A magnified image from the WT auditory cortex is shown indicating individual Tom⁺-labeled cells (red arrows), Fos⁺-labeled cells (purple arrows), and Tom⁺/Fos⁺-double-labeled cells (white arrows). n = 18 hemisphere WT-h, n = 44 hemisphere WT, and n = 24 hemisphere EphB2^−/−. For Tom⁺-single positive neurons, one-way ANOVA, F_(2,83) = 8.66, p = 0.0004; Tukey multiple comparison test; WT-h vs WT q₍₆₀₎ = 5.55, p = 0.0005; WT-h vs EphB2^−/− q₍₄₀₎ = 5.05, p = 0.0017; WT vs EphB2^−/− q₍₆₆₎ = 0.09, p = 0.9979. For Fos⁺-single positive neurons, one-way ANOVA, F_(2,83) = 22.61, p < 0.0001; Tukey multiple comparison test; WT-h vs WT q₍₆₀₎ = 9.48, p < 0.0001; WT-h vs EphB2^−/− q₍₄₀₎ = 5.44, p = 0.0007; WT vs EphB2^−/− q₍₆₆₎ = 3.77, p = 0.0248. For Tom⁺/Fos⁺-double positive neurons, one-way ANOVA, F_(2,83) = 21.14, p < 0.0001; Tukey multiple comparison test; WT-h vs WT q₍₆₀₎ = 9.17, p < 0.0001; WT-h vs EphB2^−/− q₍₄₀₎ = 6.36, p < 0.0001; WT vs EphB2^−/− q₍₆₆₎ = 2.30, p = 0.2413. Error bars are standard error of the mean (SEM); *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Scale bar = 100 μm