Fig. 5.

Analysis of Tom⁺ and Fos⁺ learning-associated neurons in the amygdala of EphB2 mutant mice. Neurons activated exclusively following encoding (Tom⁺ single-positive), exclusively following sound-cued retrieval (Fos⁺ single-positive), and following both encoding and retrieval (Tom⁺/Fos⁺ double-positive) were imaged and counted in the cortical, central, and basolateral amygdala from EphB2^−/− mutants and WT littermates subjected to FC and WT home cage controls not subjected to FC (WT-h). Scatter plots show the number of Tom⁺ single-positive neurons, Fos⁺ single-positive neurons, and Tom⁺/Fos⁺ double-positive neurons. Representative confocal images of Tom⁺ and Fos⁺ labeled neurons in the three amygdala regions are shown with the circle indicating quantification area. n = 10 hemisphere WT-h, n = 28 hemisphere WT, and n = 8 hemisphere EphB2^−/− for all amygdala regions. For Fos⁺-single positive neurons in cortical amygdala, one-way ANOVA, F_(2,43) = 11.65, p < 0.0001; Tukey multiple comparison test; WT-h vs WT q₍₃₆₎ = 6.81, p < 0.0001; WT-h vs EphB2^−/− q₍₁₆₎ = 3.56, p = 0.0406; WT vs EphB2^−/− q₍₃₄₎ = 2.05, p = 0.3253. For Tom⁺/Fos⁺-double positive neurons in cortical amygdala, one-way ANOVA, F_(2,43) = 4.89, p = 0.0123; Tukey multiple comparison test; WT-h vs WT q₍₃₆₎ = 4.37, p = 0.0096; WT-h vs EphB2^−/− q₍₁₆₎ = 1.95, p = 0.3619; WT vs EphB2^−/− q₍₃₄₎ = 1.71, p = 0.4539. For Fos⁺-single positive neurons in central amygdala, one-way ANOVA, F_(2,43) = 4.30, p = 0.0199; Tukey multiple comparison test; WT-h vs WT q₍₃₆₎ = 3.83, p = 0.0259; WT-h vs EphB2^−/− q₍₁₆₎ = 3.50, p = 0.0448; WT vs EphB2^−/− q₍₃₄₎ = 0.63, p = 0.8975. For Fos⁺-single positive neurons in basolateral amygdala, one-way ANOVA, F_(2,43) = 24.76, p < 0.0001; Tukey multiple comparison test; WT-h vs WT q₍₃₆₎ = 9.95, p < 0.0001; WT-h vs EphB2^−/− q₍₁₆₎ = 6.00, p = 0.0003; WT vs EphB2^−/− q₍₃₄₎ = 2.04, p = 0.3283. Error bars are standard error of the mean (SEM); *p < 0.05, ***p < 0.001, ****p < 0.0001. Scale bar = 50 μm