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. 2019 Oct 11;9:14708. doi: 10.1038/s41598-019-51293-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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L. reuteri effects on gut microbiota. Twelve week old C57BL/6 and Rag KO male mice were supplemented with water or L. reuteri for 4 weeks as indicated in Fig. 2. Fecal samples were collected at the day of harvest and analyzed by 16S rRNA sequencing as described in the methods. (A) Plots of alpha-diversity metrics of observed operational taxonomic units (OTUs) (richness) and Shannon diversity (richness and evenness) of the different treatment groups. (B) Principle coordinate analysis of beta-diversity based on Bray-Curtis (richness and evenness) of the different treatment groups. WT/C = wild-type control, WT/LR = wild-type L. reuteri treated, KO/C = Rag1 KO control, KO/LR = Rag1 KO L. reuteri treated. N = 9–10/group.