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. 2019 Oct 11;9:14708. doi: 10.1038/s41598-019-51293-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of live and heat killed L. reuteri 6475 on cytokine expression in whole MLN cultures. Mesenteric lymph nodes were isolated from male C57BL/6 mice (12–18 weeks), homogenized and cultured with live or heat killed L. reuteri 6475 at an MOI of 1, 10 or 100 for 4 days. Cells were analyzed using flow cytometry for expression of IL-10, INFγ, IL-17 A and TGFβ. Results for MFI and % positive cells were analysed as (A) the whole MLN or (B) gated on the MLN CD4⁺ T cells. Statistical analysis performed by 2-way ANOVA with Bonferroni’s multiple comparison test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 compared to control. n = 5.