Fig. 4.

Ncf1* mice show similar muscle size, fiber-type composition, mitochondrial content and capillary density compared to WT group. a Fiber size-related parameters in WT and ncf1* mice were determined using wheat germ agglutinin (WGA) staining. b Myofiber cross-sectional area, and c minimum (min.) Ferret diameter. d Quantification and e representative merged image of fiber-type staining where Type I fibers (blue), Type IIa (green), Type IIb (red), Type IIx (non-stained) in WT (n = 6), and ncf1* (n = 5) TA muscles. Subunits of mitochondrial oxidative phosphorylation complexes were determined in f quadriceps and g soleus muscle lysates (n = 14 per group). h Capillary density was estimated by PECAM immunostaining in tibialis anterior sections. Two-way ANOVAs was performed to test for effects of exercise (Exer), genotype (Geno), and interaction (Int), followed by Tukey’s post hoc test with correction for multiple comparisons. Individual values and mean ± SEM are shown. Scale Bar = 100 µm. For b, c, d, and h, source data and p values are provided in the Source Data file. For f and g, uncropped blots are provided in the Source Data file