Fig. 4.

HINT1 polymerizes in stimulated RBL cells. a Western blottings of the endogenous HINT1 following by the IgE and antigen stimulation. 0 indicates the unstimulated state. Source data are provided as a Source Data file. b–g Endogenous HINT1 in Rat basophilic leukemia (RBL) cell following the IgE and antigen stimulation (0–4 h) were immunostained and visualized by confocal laser scanning microscope or stimulated emission depletion microscope (STED). Scale bars, 10 μm. Nuclei were labeled with DAPI. One representative experiment out of three is shown. h The transcript level of c-Met and c-Kit following the IgE and antigen stimulation. Error bars represent the SEM of three experimental repeats. Source data are provided as a Source Data file. i Schematic cartoon showing Ap₄A induces the formation of HAn polymer to release MITF for transcriptional activity in allergic response