Fig. 1.

Snap29 mRNA is ubiquitously expressed and CRISPR-mediated targeting of exon 2 depletes SNAP29 protein. a Antisense (left) and sense (right) probe of Snap29 mRNA expression at E12.5. b CRISPR design and the resulting targeted alleles, one with a 454 bp and one with a 517 bp deletion. c Western blot analysis of skin preps of mice carrying either wild-type, heterozygous, or homozygous mutant for the Snap29 allele. d Quantification of SNAP29 expression. SNAP29 levels are normalized to WT. Lb limbud, fore, or hind, l lung, hrt heart, s somite, tb tailbud. Error bar represent: standard deviation. Scale bar equals 1000 µm