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. 2019 Oct 11;2:375. doi: 10.1038/s42003-019-0601-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 8 — Histological analysis of Snap29^−/− testis. Testis sections were stained with hematoxylin and eosin to evaluate spermatogenesis in WT (a, c) and Snap29^−/− (b, d) mice. WT testis displayed normal spermatogenesis (Stages VII and VIII showing elongating spermatids and spermatozoa in the lumen). Seminiferous tubules in Snap29^−/− testis had degenerated germ cells (white arrows), loss of immature germ cells accumulated in the lumen (arrow heads), giant multinucleated spermatids (black arrow) and extensive vacuolization (*). The diameter of degenerated seminiferous tubules was reduced in Snap29^−/− (d) compared with WT (c) testis