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. 2019 Oct 11;9:14702. doi: 10.1038/s41598-019-50976-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Increased liver steatosis characterizes CD40^fl/flCD11c^cre mice after 18 weeks of HFD. (A) Liver weight (grams). (B) Total cholesterol/gram protein and TG/gram protein of liver. (C) Histopathological scoring of liver steatosis and representative H&E staining of liver. (D) Quantification of liver Oil-red-O (ORO) staining and representative pictures. (E) Plasma liver enzymes: alanine aminotransferase (ALT) activity, aspartate aminotransferase (AST) levels, albumin levels and glutamate dehydrogenase (GDH) activity. Data is represented as mean ± SEM. *P < 0.05, **P < 0.01, ***p < 0.001 for comparison between WT and CD40^fl/flCD11c^cre mice fed the same diet. n = 7/group for SFD, and n = 8/group for HFD.