Table 1.
Peptide and fluorophore-peptide conjugate description
| Peptide Abbreviation | Amino acid sequence | Molecular mass (gmol−1) | Purity (%) |
|---|---|---|---|
| GYR | GYRPVHNIRGHWAPG | Not applicable | – |
| CGY | CGYRPVHNIRGHWAPG | 1820.04 | 98.11 |
| FAM-CGY | 5-FAM-CGYRPVHNIRGHWAPG | 2178.36 | 98.91 |
| CGY-(K)-FAM | CGYRPVHNIRGHWAPGK-5-FAM | 2305.53 | 98.36 |
| FAM-GYR | 5-FAM-GYRPVHNIRGHWAPG | 2075.24 | 98.46 |
| FAM-CGY scrambled 1 | 5-FAM-CGYRPVHNIGHWRAPG | 2177.36 | 98.56 |
| FAM-CGY scrambled 2 | 5-FAM-CGYRPVHNIRGHGAPG | 2048.20 | 98.26 |
| Cy5.5-CGY | Cy5.5-CGYRPVHNIRGHWAPG | 2385.30 | 98.20 |
| Cy5.5-CGY scrambled 1 | Cy5.5- CGYRPVHNIGHWRAPG | 2385.90 | 98.50 |
Sequences are represented by one standard amino acid code symbols. 5-FAM = 5-carboxy-fluorescein and Cy5.5 = cyanine 5.5