Fig. 1.

Mouse model with sustained elevation of MG53 in the bloodstream. a 1 µl sera derived from 3-month wild type (WT) and tPA-MG53 mice at 2 months (young), 12 months (middle) and 24 months (aged) were probed with anti-MG53 antibody. b Quantification of serum levels of MG53 in wild type and tPA-MG53 mice by western blot (n = 10, wild type; n = 9 for tPA-MG53, P < 0.0001). c Skeletal muscle (2 µg per lane) and heart (2 µg per lane) derived from tPA-MG53 and WT littermate mice were blotted with anti-MG53 antibody. Red arrows indicate doublet of MG53 and tPA-MG53. d H&E staining of vital organs from 32-month-old tPA-MG53 mice show normal tissue morphology. The pictures are representative of two other tPA-MG53 mice at 32 months age. Error bar represents the standard deviation and P value was generated by t test