Fig. 5.

tPA-MG53 skeletal muscle showed enhanced regeneration capacity after cardiotoxin injury. a H/E staining of gastrocnemius muscle derived from KO, WT, and tPA-MG53 mice at 7 days post cardiotoxin injury. Enlarged pictures show different regenerative capacity of muscle fibers derived from WT, KO, and tPA-MG53 mice. b IHC staining of MG53 (red) and mouse IgG (green). IgG positive staining, mostly in KO muscle, indicates necrotic muscle fibers after cardiotoxin injury. c IHC staining with antibody against CD11b to show the different degrees of the presence of immune cells at the muscle injury sites (7 days post cardiotoxin treatment). d Quantification of muscle fiber size (left), IgG staining of necrotic fibers (middle) and number of cells positive for CD11b (right). ***p < 0.001, **p < 0.01. Error bar represents the standard deviation and p value was generated by t test