Fig. 6.

MG53 modulates muscle satellite cell (mSC) proliferation to contribute to muscle regeneration. a Outgrowth of mSCs from isolated EDL muscle fibers derived from adult wild-type, tPA-MG53 and KO mice. At day 5 after culture, there were fewer mSCs growing out from KO muscle fibers, as compared to those from wild type and tPA-MG53 muscle fibers. Incubation with exogenous rhMG53 (20 μg/ml) led to increased number of mSCs near the KO myofiber (KO + rhMG53). b Flow cytometry analysis with different cell lineage antibodies (Pax7 for mSCs, MyoD for myoblasts, and PDGFα for fibroblasts) to validate the identity of the cultured mSCs. c Immunofluorescent staining confirmed the high purity of muscle-fiber derived mSCs, as they are positive for Pax7 and negative for MyoD. d Statistical analysis demonstrate that the compromised proliferation of mSCs derived from KO muscle could be restored by incubation with rhMG53 (n = 3–6 per condition, *p < 0.05 **p < 0.01 as compared to mg53−/− fibers). Error bar represents the standard deviation and p value was generated by t test