Fig. 8.

Cross of tPA-MG53 mice with db/db mice did not alter the diabetic phenotype. a Growth patterns of WT and tPA-MG53 littermates; and db/db and db/db-tPA-MG53 littermates were followed during the period of 3–32 weeks, under normal diet conditions. b GTT tests were conducted with the four groups of mice at 18 weeks of age (left). ITT tests were conducted at 20 weeks of age. No significant differences were observed among the WT and tPA-MG53, and db/db and db/db-tPA-MG53 groups, respectively. n = 6 for WT, n = 5 for tPA-MG53, n = 6 for db/db, and n = 5 for db/db-tPA-MG53. c Echocardiogram shows similar ejection fraction and fractional shortening between db/db-tPA-MG53 and db/db littermates. d Western blot of soleus muscle derived from the four groups of mice. Expression levels of IRS-1, IR-β, and PPARα were probed with the respective antibodies. α-actinin serves as loading control. Error bar represents the standard deviation