Fig. 5.

Changes in the vascular cell surface proteome in a murine model of MRSA-sepsis. Biotinylation perfusions were coupled to an automated shotgun proteomics workflow to identify organ-specific vascular targets changing in a murine model of MRSA-sepsis. We performed 4 separate experiments, which resulted in the simultaneous proteomics profiling of infected (n = 12) and uninfected (n = 12) mice across 5 major organs (liver, kidney, heart, brain, and WAT). Differential expression analysis of proteins significantly changing during infection showed that the examined organs displayed a clear hierarchy in terms of the type and amounts of vascular proteome that was altered during sepsis, with liver samples being among the most severely affected. Principal component analysis (PCA) of the identified proteins segregated the liver, kidney and brain tissues into infected and uninfected groups, but was less specific for WAT and heart stratification (b). Unsupervised hierarchical clustering of the data revealed dramatic proteome changes at 24 h post-infection with large protein clusters being differentially regulated across all examined organs (c)