Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Oct 11;10:4632. doi: 10.1038/s41467-019-12610-x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 3 — ZC3H18 depletion causes hypermethylation of the BRCA1 promoter. a Map of the divergent BRCA1/NBR2 promoter with CpG islands indicated by circles below the map. Methylation patterns obtained by bisulfite sequencing of 10 individual clones of PCR products from genomic DNA of control luciferase (Luc) and ZC3H18 siRNA (siZC3)-transfected OVACR-8 cells. Methylated (filled circles) and unmethylated (open circles) CpG positions are shown. b ChIP assays showing increased DNMT1 occupancy on the BRCA1 promoter in ZC3H18-depleted cells. OVCAR-8 (top panel) and PEA1 (bottom panel) cells transfected with control luciferase (Luc) or ZC3H18 siRNAs were harvested 48 h after transfection and processed for ChIP to detect DNMT1 on the BRCA1 promoter. c and d OVCAR-8 (c) and PEA1 (d) cells were transfected with control (Luc) or ZC3H18 siRNAs and treated with vehicle or 5-aza-2′-deoxycytidine (5 μM) for 3 days. BRCA1 mRNA (top panel) and ZC3H18 mRNA (bottom panel) levels were analyzed by qRT-PCR. The mRNA levels are normalized to GAPDH mRNA levels as the internal control. Data are means ± SEM from three independent experiments in b–d. *p < 0.05, **p < 0.01, ***p < 0.001, unpaired Student’s t-test