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. 2019 Oct 15;30(22):2814–2826. doi: 10.1091/mbc.E19-07-0364

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© 2019 Haribowo et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

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FIGURE 7: — The rate of DoxSa accumulation determines the level of DoxDHCer accumulation. Levels of C₂₆-DoxDHCer (A) and total DoxDHCer (B) in WT and the CerS3 strain following the standardized DoxSa treatments (indicated concentrations of DoxSa, 1 million cells/ml, 1.5 h) determined by MS; n = 3. (C, D) Levels of C₂₆-DoxDHCer (C) and total DoxDHCer (D) in the CerS3 strain monitored by a time-course lipid analysis by MS following a DoxSa treatment at 6 μM for up to 3 h; n = 2. (E) Effect of DoxSa on the growth of the indicated strains evaluated by a spot assay. Deletion of ceramidase (Δypc1 Δydc1) did not modulate the sensitivity of the CerS3 strain to DoxSa, indicating that the accumulation of DoxDHCer is independent of ceramidase.