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. 2019 Oct 11;9:14695. doi: 10.1038/s41598-019-51193-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of metformin pretreatment on the protein expression of AMPK and ACC in the liver of mice infected with P. chabaudi. Representative immunoblots and quantification of phosphorylation levels of AMPK (A) and ACC (B). Images represents cropped blots of the same membrane. Cropped slices are showed on Supplementary Information (S2). White bars indicating control (CTR, n = 9), black bars indicate infected (INF, n = 9), light gray bars indicate metformin pretreated control (CTR-Met, n = 9), and dark gray bars indicate metformin pretreated infected (INF-Met, n = 7) groups. Statistical analyses: one-way ANOVA followed by Dunnett’s multiple comparisons test. Values are presented as mean ± standard error. ^***p < 0.001.