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Overexpression of miR-27b inhibit cancer stem cells proliferation. Stem cells were transfected with miR-mimic NC, miR-27b mimics, miR-inhibitor NC and miR-27b inhibitor, respectively. A. The expression of miR-27b were evaluated by RT-qPCR. B. Cell Counting Kit-8 assay was used to detect viability of stem cells. C. Colony formation assay was performed to evaluate the ability of proliferative activity of HT29 stem cells. *P < 0.05, ***P < 0.001 vs. miR-NC.
