Figure 1.

Increased fasting blood-glucose (FBG), 24-hour urine protein level, renal tissue injury, renal fibrosis, and collagen IV were demonstrated in kidney of DN rats in comparison with control normal rats by methods of an automatic biochemistry analyzer, HE staining, Masson staining and immunofluorescence. A. The broken line graph of FBG and 24-hour urine protein level in kidney of DN rats and control normal rats. B. HE staining for renal tissue injury in kidney of DN rats and control normal rats (magnification, ×200). C. Masson staining for renal fibrosis in kidney of DN rats and control normal rats (magnification, ×200). D. Immunofluorescence of collagen IV (indicated in green) with DAPI counterstaining in control normal rats and DN rats. n = 9. ***P < 0.001 versus control group.