Increased PTEN, FN, collagen I, IV, p62, decreased LC3II and beclin1 were revealed in DN rats and control normal rats. A. Western blot detection and statistical analysis for PTEN, FN, collagen I and IV proteins in kidney of control normal rats and DN rats. B. Immunofluorescence of LC3II (indicated in green) with DAPI counterstaining in control normal rats and DN rats. C. Western blot of beclin1 and p62 proteins expression in kidney of control normal rats and DN rats. n = 9. **P < 0.01, ***P < 0.001 versus control group.