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. 2019 Sep 15;11(9):5472–5486.

Figure 3.

Figure 3

Increased IL-6, TNF-α, IL-1β, NO, iNOS and ANGPTL2 were showed in in DN rats and control normal rats. A. ELISA detection and statistical analysis for serum IL-6, TNF-α and IL-1β in control normal rats and DN rats. B. Griess reaction for NO concertration in in kidney of control normal rats and DN rats. C. Western blot detection and statistical analysis for iNOS in kidney of control normal rats and DN rats. D. Real-time qPCR of ANGPTL2 mRNA with an internal control of GAPDH in kidney of control normal rats and DN rats. E. Western blot of ANGPTL2 protein expression in kidney of control normal rats and DN rats. F. The level of serum ANGPTL2 was detected by method of ELISA in normal or DN rats. G. Immunohistochemistry of ANGPTL2 expression in kidney of control normal rats and DN rats. N = 9. *P < 0.05, **P < 0.01, ***P < 0.001 versus control group.