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. 2019 Sep 15;11(9):5472–5486.

Figure 6.

Figure 6

The effects of knocking down of ANGPTL2 and U0126 on p62, beclin1, IL-6, TNF-α, IL-1β, and NO in HG-induced HK-2 cells. A. Expression of p62 and beclin1 were determined by Western blot and quantified by densitometry in HK-2 cells of control normal group (control), high glucose group (HG), high glucose plus transfected with si-ANGPTL2-1 vector group (si-ANGPTL2-1+HG), and high glucose plus transfected with si-ANGPTL2-1 vector plus U0126 group (si-ANGPTL2-1+HG+U0126). B. ELISA for IL-6, TNF-α and IL-1β in in HK-2 cells of control normal group (control), high glucose group (HG), high glucose plus transfected with si-ANGPTL2-1 vector group (si-ANGPTL2-1+HG), and high glucose plus transfected with si-ANGPTL2-1 vector plus U0126 group (si-ANGPTL2-1+HG+U0126). C. Griess reaction for NO concertration in HK-2 cells of control normal group (control), high glucose group (HG), high glucose plus transfected with si-ANGPTL2-1 vector group (si-ANGPTL2-1+HG), and high glucose plus transfected with si-ANGPTL2-1 vector plus U0126 group (si-ANGPTL2-1+HG+U0126). Each experiment was repeated three times. ###P < 0.001 versus control group; **P < 0.01, ***P < 0.001 versus HG group; ΔP < 0.05, ΔΔP < 0.01, ΔΔΔP < 0.001 versus si-ANGPTL2-1+HG group.