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. 2019 Sep 15;11(9):5457–5471.

Figure 8.

Figure 8

Overexpression of FOXD2-AS1 partially reverses the suppressive effect of miR-185-5p in TPC cells. BCPAP or TPC-1 cells were transfected with miR-185-5p mock or miR-185-5p mimics, with or without FOXD2-AS1 overexpression vector. A. Cell proliferation of BCPAP or TPC-1 was analyzed at indicated time points by CCK-8 kit. B, C. Colony formation and cell invasion of BCPAP cells were analyzed by colony formation assay and transwell assay, respectively. D. The protein expression of Cyclin D2, Wnt1, and TGFβR2 in BCPAP cells transfected with miR-185-5p mock or miR-185-5p mimics, with or without FOXD2-AS1 overexpression vector was analyzed by western blot. Data are presented as the mean ± SD; Using the Student’s t-test for statistical analysis. *P<0.05 and **P<0.01.