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Decreased level of Nrg4 in serum and renal tissue of DN rats. Total protein and DNA was extracted from the renal tissue and serum. The level of Nrg4 was detected by Western blot analysis (A), RT-qPCR (B), Immunohistochemistry (C). The data are presented as the means ± SEM, n = 3. ***P < 0.001, vs. Control (serum) group. ###P < 0.001, vs. Control (tissure) group.
