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. 2019 Sep 15;11(9):5501–5513.

Figure 7.

Figure 7

Nrg4 ameliorates fibrosis in HK-2 cell via TNF-R1 signaling. A, B. The TNF-R1 expression level was analyzed by Western blot and RT-qPCR. C. The expression levels of PTEN, FN, Collagen I and Collagen IV protein were detected by Western blot analysis. D. Immunofluorescence was used to measure the Collagen IV levels. The columns and error bars represent the mean and SEM. ***P < 0.001, vs. Control group. ##P < 0.01, ###P < 0.001, vs. HG group. ΔP < 0.05, ΔΔΔP < 0.001, vs. HG+Nrg4+pcDNA group.