Figure 8.

Nrg4 attenuate the expression of advanced glycosylation end products and inflammation in HK-2 cell via TNF-R1 signaling. (A) The levels of AGEs, RAGEs and S100B were detected by Western blot analysis. (B) Immunofluorescence was used to measure the AGEs levels. The levels of TNF-α (C), IL-6 (D) were measured using appropriate enzyme-linked immunoassay (ELISA) kits. The levels of iNOS (E) and NO (F) were using commercial test kits. The data are presented as the means ± SEM. ***P < 0.001, vs. Control group. ##P < 0.01, ###P < 0.001, vs. HG group. ΔΔP < 0.01, ΔΔΔP < 0.001, vs. HG+Nrg4+pcDNA group.