Blnc1 interference significantly decreased the ROS level in HG-induced HK-2 cells. The effect of Blnc1 on the ROS level in HK-2 cells treated with D-glucose. The ROS level was assessed with 2’, 7’-Dichlorodihydrofluorescein diacetate. HK-2 cells were either untreated, or treated with D-glucose, and transfected with control or Blnc1 inhibitor. **P < 0.01, ***P < 0.001 vs. control group, ### P < 0.001 vs. HG group.