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. 2019 Sep 15;11(9):5956–5966.

Figure 4.

Figure 4

3MA abolished the protective effect of sinomenine against TBHP in rat NP cells. A. CCK-8 assay was performed to evaluate the proliferation of rat NP cells treated with 3.33 mM sinomenine + 100 μM THBP or 3.33 mM sinomenine + 100 μM THBP + 5 mM 3MA, respectively. B, C. Annexin V/PI assay was performed to evaluate the apoptosis rate in NP cells treated with 3.33 mM sinomenine + 100 μM THBP or 3.33 mM sinomenine + 100 μM THBP + 5 mM 3MA, respectively. D, E. Western blotting assay was performed to measure the protein level of active caspase 3 in NP cells treated with 3.33 mM sinomenine + 100 μM THBP or 3.33 mM sinomenine + 100 μM THBP + 5 mM 3MA, respectively. N = 3, ##P < 0.01 vs. 3.33 mM sinomenine + 100 μM THBP.