Figure 2.

Induced THP-1 macrophages showed the typical functional phenotype of M2-polarized macrophages. A. Images of THP-1 cells and M2-polarized macrophages. B. THP-1 cells treated with PMA showed significant induction of CD206 and CD204 expression (both markers of M2 macrophages). An ELISA also showed that induced macrophages possessed M2-type macrophage-like secretory functions, including significantly lower levels of TNF-α and higher levels of TGF-β, than those seen with coculture with THP-1 cells (P<0.05).