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. 2019 Sep 15;11(9):6055–6065.

Figure 4.

Figure 4

The impacts of combined inhibitors of PI3K and CDK4/6 on PIK3CA mutated cells. A. A dose matrix of PI3K inhibitor BKM120 and CDK 4/6 inhibitor Palbociclib was performed in cell lines transfected with mutant PIK3CA. Viability was assessed one day after treatment. The concentrations and percentages of inhibition at each dose of drug were presented. B. Colony formation of cells transfected with mutant PIK3CA were carried out after treatments of single BKM120 alone or with Palbociclib. C. The representative images of cell apoptosis were analyzed by flow cytometry in cells transfected with wild-type or mutant PIK3CA following medication. D. The representative images of cell cycle were analyzed by flow cytometry in cells transfected with mutant PIK3CA after exposure to BKM120 alone or with Palbociclib for 24 hours. Percentages of cells in G1, S, and G2 phases were quantified. *P < 0.05.