Table A3.
Clinical pathology evaluated by absolute mean values (103/µL) obtained in the counts of leukocytes, platelets and serum iron of animals vaccinated with vaccines against BVDV and BHV-1 with different commercial formulations.
| Variables | First Dose | Second Dose | |||||||
|---|---|---|---|---|---|---|---|---|---|
| Vaccine A 2 | Vaccine B 3 | Vaccine C 4 | Control 5 | Vaccine A 2 | Vaccine B 3 | Vaccine C 4 | Control 5 | ||
| WBC 6(103/µL) | 6 h 1 | 16.63 | 17.22 a | 16.07 | 13.47 | 15.89 A,b,a | 15.80 A,B,a | 18.36 A,a | 13.50 B,a |
| 24 h | 14.64 | 13.66 b | 16.04 | 13.60 | 13.84 a | 12.58 b | 14.40 b | 11.80 a,b | |
| 48 h | 14.43 | 12.19 b | 14.13 | 11.63 | 13.73 a | 12.23 b | 14.36 b | 11.45 a,b | |
| 72 h | 13.92 | 13.41 b | 14.30 | 12.08 | 13.51 a | 12.21 b | 14.11 b | 10.60 a,b | |
| 168 h | 13.70 | 13.21 b | 14.30 | 14.05 | 12.07 A,B,b | 12.20 A,B,b | 13.45 A,b | 9.62 B,b | |
| LYMPH 7(103/µL) | 6 h | 7.76 | 7.01 | 6.58 | 6.98 | 8.69 | 6.99 | 6.73 | 7.42 |
| 24 h | 8.43 | 6.91 | 6.33 | 6.13 | 8.65 | 7.01 | 5.96 | 6.65 | |
| 48 h | 8.25 | 6.59 | 7.39 | 6.48 | 8.40 | 6.51 | 7.08 | 6.71 | |
| 72 h | 8.09 | 7.37 | 7.25 | 6.82 | 8.45 | 6.96 | 7.22 | 5.98 | |
| 168 h | 8.11 | 7.57 | 7.67 | 7.45 | 7.78 | 6.71 | 8.19 | 5.93 | |
| MONO 8(103/µL) | 6 h | 2.11 a | 1.80 | 1.72 a,b | 2.06 a | 1.40 | 1.32 | 1.52 | 1.58 |
| 24 h | 2.06 a | 1.81 | 1.99 a | 2.22 a | 1.00 | 1.21 | 1.14 | 0.97 | |
| 48 h | 1.86 a,b | 1.44 | 1.71 a,b | 1.43 b | 1.31 | 1.23 | 1.25 | 1.09 | |
| 72 h | 1.85 a,b | 1.35 | 1.59 a | 1.23 b | 1.35 | 1.24 | 1.51 | 1.05 | |
| 168 h | 1.31 b | 1.38 | 1.24 b | 1.31 b | 1.02 | 1.17 | 1.33 | 0.76 | |
| BAS 9(103/µL) | 6 h | 0.68 A,B | 0.68 A,B | 0.41 B | 1.19 A | 0.73 | 0.90 | 0.60 | 1.11 |
| 24 h | 0.86 | 0.94 | 0.84 | 1.36 | 1.01 | 1.10 | 1.28 | 1.27 | |
| 48 h | 0.53 | 0.74 | 0.98 | 1.02 | 1.19 | 1.25 | 1.29 | 0.70 | |
| 72 h | 0.29 | 0.80 | 0.65 | 0.99 | 1.00 | 1.08 | 1.0 | 0.89 | |
| 168 h | 0.46 | 0.70 | 0.63 | 0.69 | 0.87 | 0.68 | 0.89 | 0.65 | |
| NEUTR 10(103/µL) | 6 h | 5.80 A,B,a | 7.38 A,a | 7.28 A,a,b | 3.26 B,a,b | 5.17 B,C,a | 6.54 B,a | 9.10 A,a | 3.43 C,a,b |
| 24 h | 3.21 B,b | 3.80 B,b | 6.92 A,b | 3.60 B,a,b | 2.92 B,b | 3.50 B,b | 5.83 A,b,c | 2.84 B,a,b | |
| 48 h | 3.70 A,b | 3.16 A,b | 3.87 A,c | 2.63 A,b | 2.91 A,b | 3.20 A,b | 4.34 A,c | 2.94 A,a,b | |
| 72 h | 3.54 A,B,b | 3.47 A,B,b | 4.68 A,c | 2.54 B,b | 2.63 B,b | 3.16 A,B,b | 4.17 A,c | 2.62 B,b | |
| 168 h | 3.68 A,b | 3.39 A,b | 4.66 A,c | 4.60 A,b | 2.49 A,B,b | 3.70 A,B,b | 3.92 A,c | 2.16 B,b | |
| EOS 11 (103/µL) | 6 h | 0.54 B,a,b,c | 0.60 B | 0.42 B,b | 1.08 A,a,b | 0.75 a,b,c | 0.78 | 0.96 a,b | 0.96 a,b |
| 24 h | 0.72 B,a,b,c | 0.78 B | 0.74 B,a,b | 1.40 A,a | 1.04 a | 0.98 | 1.39 a | 1.12 a,b | |
| 48 h | 0.45 b,c | 0.65 | 1.10 a,b | 1.00 a,b | 1.08 a | 0.99 | 1.44 a | 0.60 b | |
| 72 h | 0.29 B,c | 0.73 A,B | 0.70 B,a,b | 1.40 A,a | 0.98 a,b | 0.99 | 1.06 a,b | 0.78 a,b | |
| 168 h | 0.40 b,c | 0.59 | 0.68 a,b | 0.57 b | 0.78 a,b,c | 0.68 | 0.81 a,b | 0.60 b | |
| PLAT 12(×103/µL) | 6 h | 214.33 A,B | 333.30 A | 254.20 A,B | 168.17 B | 246.56 | 221.00 | 208.80 | 188.00 |
| 24 h | 263.67 | 251.60 | 235.20 | 251.33 | 225.33 | 227.30 | 209.10 | 155.67 | |
| 48 h | 205.67 | 275.60 | 193.70 | 259.67 | 204.89 | 228.60 | 191.50 | 178.33 | |
| 72 h | 220.00 | 253.70 | 285.00 | 264.67 | 219.67 A,B | 241.00 A | 201.60 A,B | 157.17 B | |
| 168 h | 238.11 | 266.00 | 235.00 | 252.50 | 207.89 | 246.00 | 237.70 | 163.00 | |
| Iron (µM/L) | 0 h | 28 A | 26 A,B | 23 B | 32 | 23 A,b | 27 A,a,b | 25 A,a | 29 A,a,b |
| 6 h | 27 A,B | 26 B | 21 B | 35 | 20 A,B,b | 16 B,c | 14 B,b | 25 A,b | |
| 24 h | 29 A,B | 23 B | 15 C | 32 | 21 A,B,b | 19 B,b,c | 12 C,b | 30 A,a,b | |
| 48 h | 30 A,B | 26 A,B | 23 B | 32 | 33 A,b,a | 27 B,a,b | 14 C,b | 38 A,a | |
| 72 h | 28 A,B | 24 B,C | 21 C | 33 | 27 A,B,a,b | 26 A,B,b | 24 B,a | 33 A,a,b | |
1 h: hours; 1h: hours; 2 Vaccine A (n = 9); 3 Vaccine B (n = 10); 4 Vaccine C (n = 10); 5 Control (n = 6); 6 WBC: Total leukocytes; 7 LYMPH: Lymphocytes; 8 MONO: Monocytes; 9 BAS basophils; 10 NEUTR: Neutrophils; 11 EOS: Eosinophils; 12 PLAT: Platelets; capital letters on the same line show difference between treatments; lowercase letters in the same column show difference between times. Differences were considered significant when p ≤ 0.05.