Figure 2.

Tests of normalization methods on simulated data. The top panels plot the Zamar criterion, which is 0 for perfect inference of the true activity. The middle panels plot the 90th percentile ($P_{90}$) of the relative bias. The bottom panels plot the relative median absolute deviation, a measure of precision. (a) Performance of the methods plotted against mean transfection efficiency. True activity: $A=10$. Sample size: $N=10$. Renilla errors: ${\sigma }_{11}={\sigma }_{12}=3$. Firefly errors were scaled with activity: ${\sigma }_{21}={\sigma }_{22}=10{\sigma }_{11}$. (b) Performance plotted against sample size. Mean transfection efficiency: $\overline{t}=0.25$. The other parameters are the same as in panel (a,c). Performance plotted against true relative activity. Sample size: $N=10$. The other parameters are the same as in panel (b).