Table 3.
Quantitative real-time PCR analysis of IL-2 expression in PBMCs of chickens challenged with very virulent, infections bursal disease virus (vvIBDV) at different days post challenge (DPC).
S. number | Group | 7 DPC | 14 DPC |
---|---|---|---|
1. | Negative control (Group A) | 1 a ± 0.00 | 1 a ± 0.00 |
2. | Positive control (Group B) | 3.977 b ± 0.200 | 2.957 b ± 0.210 |
3. | T. cordifolia + virus challenge (Group C) | 6.350 c ± 0.132 | 5.740 c ± 0.231 |
4. | CpG ODN + virus challenge (Group D) | 8.068 d ± 0.207 | 7.462 d ± 0.507 |
5. | T. cordifolia + CpG ODN + challenge (Group E) | 10.840 e ± 0.232 | 10.620 e ± 0.723 |
a–e Means bearing different superscripts column-wise differ significantly (p < 0.05). The results are expressed as fold changes to the immune response gene transcripts relative to the control group.