Table 1.
Mass numbers observed in the reactions containing individual nucleotides. Detailed mass spectra and peaks obtained for the individual nucleotide polymerization reactions are included in the Supplementary Materials File 1.
| Chemical Species | Expected Mass | Observed Mass | ppm Error |
|---|---|---|---|
| AMP Reaction | |||
| Adenine | 136.0617 | 136.0635 | 13.2290 |
| AMP monomer | 348.0703 | 348.0691 | 3.4475 |
| Abasic Dimer | 560.0778 | 560.0764 | 2.4996 |
| Abasic Trimer | 772.0874 | 772.0899 | 3.2379 |
| GMP Reaction | |||
| Guanine | 152.0566 | 152.057 | 2.6305 |
| GMP Monomer | 364.0652 | 364.0627 | 6.8669 |
| Abasic Dimer | 576.0737 | 576.0715 | 3.8189 |
| Abasic Trimer | 788.0824 | 788.0803 | 2.6646 |
| UMP Reaction | |||
| Uracil | 113.0345 | 113.0346 | 0.8846 |
| UMP Monomer | 325.0431 | 325.0435 | 1.2306 |
| Intact Dimer | 631.0684 | 631.0682 | 0.3169 |
| Abasic Dimer | 537.0517 | 537.0533 | 2.9792 |
| CMP Reaction | |||
| Cytosine | 112.0505 | 112.0494 | 9.8170 |
| CMP Monomer | 324.0591 | 324.0596 | 1.5429 |
| Intact Dimer | 629.1004 | 629.1017 | 2.0664 |
| Abasic Dimer | 536.0677 | 536.0677 | 0.0000 |