Table 2.
Peaks observed in MS analysis of mixed nucleotide reactions. Potential structures for some of the chemical species is depicted in Supplementary Figure S3 in Supplementary Materials File 2. Detailed mass spectra and peaks obtained for reactions containing base pairing nucleotides are included in the Supplementary Materials File 1.
| Chemical Species | Expected Mass | Observed Mass | ppm Error |
|---|---|---|---|
| AMP + UMP Reaction | |||
| Adenine | 136.0617 | 136.0635 | 13.2290 |
| AMP Monomer | 348.0703 | 348.0691 | 3.4475 |
| Uracil | 113.0345 | 113.0346 | 0.8846 |
| UMP Monomer | 325.0431 | 325.0435 | 1.2306 |
| Abasic A Dimer | 560.0778 | 560.0812 | 6.0705 |
| Abasic U Dimer | 537.0517 | 537.0533 | 2.9792 |
| Abasic A Trimer | 772.0874 | 772.0899 | 3.2379 |
| Abasic U Trimer | 749.0603 | 749.0637 | 4.5390 |
| GMP + CMP Reaction | |||
| Guanine | 152.0566 | 152.057 | 2.6305 |
| GMP Monomer | 364.0652 | 364.0627 | 6.8669 |
| Cytosine | 112.0505 | 112.0494 | 9.817 |
| CMP Monomer | 324.0591 | 324.0596 | 1.5429 |
| Intact CC Dimer | 629.1004 | 629.1017 | 2.0664 |
| Intact CG Dimer | 669.1065 | 669.1042 | 3.4374 |
| Abasic G Dimer | 576.0737 | 576.0715 | 3.8189 |
| Abasic C Dimer | 536.0677 | 536.0677 | 0.0000 |
| Abasic G Trimer | 788.0824 | 788.0803 | 2.6646 |
| Abasic C Trimer | 748.0763 | 748.0787 | 3.2082 |