Figure 3.

A. lappa extract reduced inflammation induced by tumor implantation. (A,C) Total leukocytes and (B,D) neutrophil accumulation was evaluated in the peritoneal cavity after 30 days of the B16F10 melanoma implantation. The animals received Alhe (50 mg/kg/300 μL) or PBS (300 μL) via i.p. every 5 days for 30 days. (E) Total protein, (F) NO₂⁻, (G) IL-6; (H) TNF-α, and (I) IL-1β were quantified in the peritoneal fluid. The experiment was conducted twice using five mice per group (n = 5). Data represents mean ± SEM. * control versus tumor or tumor + A. lappa; ^# tumor versus A. lappa. Differences were considered significant with p < 0.05 according to ANOVA with Tukey’s post-test.