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. 2019 Sep 16;8(3):152. doi: 10.3390/pathogens8030152

Figure 1.

Figure 1

Determination of the detection limits of simplex and multiplex PCR. (A) Detection of A. lumbricoides ITS1 gene. Lane L: DNA ladder; Lane 1: negative control; Lanes 2–7: 10-fold serial dilutions of gDNA of A. lumbricoides at 100, 10, 1, 0.1, 0.01, and 0.001 ng. (B) Detection of N. americanus 18s rRNA gene. Lane L: DNA ladder; Lane 1: negative control; Lanes 2–7: 10-fold serial dilutions of gDNA of N. americanus at 100, 10, 1, 0.1, 0.01, and 0.001 ng. (C) Detection of S. stercoralis 18s rRNA gene. Lane L: DNA ladder; Lane 1: negative control; Lanes 2–7: 10-fold serial dilutions of gDNA of S. stercoralis at 100, 10, 1, 0.1, 0.01, and 0.001 ng. (D) The sensitivity of multiplex PCR. Lane L: DNA ladder; Lane 1: negative control; Lanes 2–7: 10-fold serial dilutions of mixed gDNA at 100, 10, 1, 0.1, 0.01, and 0.001 ng of each parasite.