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. 2019 Sep 16;8(3):152. doi: 10.3390/pathogens8030152

Figure 2.

Figure 2

Determination of the specificity of multiplex PCR. Lane L: DNA ladder; Lane 1: negative control; Lane 2: multiplex PCR using triple plasmid controls; Lane 3: multiplex PCR using gDNA isolated from A. lumbricoides and Giardia lamblia; Lane 4: multiplex PCR using gDNA isolated from G. lamblia; Lane 5: multiplex PCR using gDNA isolated from G. lamblia and Blastocystis hominis; Lane 6: parasite-free human feces.