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. 2019 Sep 16;8(3):152. doi: 10.3390/pathogens8030152

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Multiplex PCR for simultaneous detection of soil-transmitted helminth (STH) infections using clinical samples. The amplified fragments from species-specific primers were visualized on 1% agarose gel using ethidium bromide staining. Lane L: DNA ladder; Lane 1: S. stercoralis; Lane 2: A. lumbricoides; Lane 3: N. americanus; Lane 4: S. stercoralis and A. lumbricoides; Lane 5: N. americanus and A. lumbricoides; Lane 6: S. stercoralis and N. americanus; and Lane 7: S. stercoralis, A. lumbricoides, and N. americanus.