Fig. 5.

Changes of free FA and the downstream metabolites 19,20-EpDPA and 19,20-DiHDPA in peritoneal lavages in the zyA-induced peritonitis model, with and without SH42 treatment. For all experiments, n = 6 to 10; data were obtained from 2 independent experiments with an individual n = 3 to 5 (single experiment for PGE₂ with n = 4 to 5). *P < 0.05, **P < 0.01; nonparametric Student’s t test. (A–H) Longitudinal behavior of various FAs during zyA-induced peritonitis with and without SH42 treatment (nonparametric Student’s t test [Mann–Whitney]). Ctrl, control. (I) Chromatographic trace showing the transition m/z 343→281 was used to detect 19,20-EpDPA. AU, arbitrary units. (J) Enzymatic formation of 19,20-EpDPA and 19,20-DiHDPA catalyzed by CYP450 and soluble epoxide hydrolase. (K and L) Levels of MaR1 and PGE₂ observed at the 4-h and 12-h time points. Results are expressed as mean ± SEM. *P < 0.05, **P < 0.01; nonparametric Students t test (Mann–Whitney). (M) MS/MS spectra identifying 19,20-EpDPA compared with an authentic standard (1 ng/mL, blue boxed inlay).