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. 2019 Jul 16;7(3):64. doi: 10.3390/vaccines7030064

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Cell-mediated immunity against influenza antigens after A/H1N1/California.pdm09 challenge was shown as IFN-gamma responses in stimulated spleen cells in vitro in three study groups of vaccinated NMRI mice (Figure 10A–C). (A) illustrates the GMT (95% C.I) IFN-gamma ELIspot responses in WIV vaccinated mice (no adjuvant) at day the day of sacrifice day 9–18, (B) the WIV with N3 as adjuvant, at the day of sacrifice, at days 12–30, and (C) the WIV with N3 and FliC-DNA as adjuvant, at day of sacrifice, at day 29–30. The frequencies of spots/million were evaluated against WIV/Influenza A/H1 and A/H3, as well as against two CTL-peptides from the NP-protein. Significant differences are indicated by nonparametric Mann–Whitney U analysis p-values.