Figure 11.

Cell-mediated immunity against influenza antigens after challenge was shown as IFN-gamma responses in stimulated spleen cells in vitro in three study groups of vaccinated C57BL6 mice (A–C). (A) illustrates the GMT (95% C.I) IFN-gamma ELIspot responses in WIV vaccinated mice (no adjuvant) at day of sacrifice at days 6, 9, and 12, (B) the WIV with N3 as adjuvant, at day of sacrifice at days 9, 18, and 30 and (C) the WIV with N3 and FliC-DNA as adjuvant, at day of sacrifice at days 12, 21, and 30. Frequencies of spots/million were evaluated against WIV/Influenza A/H1 and A/H3, as well as against one CTL-peptide from the NP-protein. Significant differences are indicated by nonparametric Mann–Whitney U analysis p-values.