Table 1.
Study design and nasal immunization of NMRI and C57BL/6 mice against formalin-inactivated Influenza A/H1N1/Salomon Island/2006.
| Immunization Schedule | |||||
|---|---|---|---|---|---|
| Group | n | Immunization Days | Dose and Immunogen | Adjuvant | Mouse Strain |
| 1 | 32 | 0, 60 | 1.5 µg HA in whole intact inactivated H1N1 | None | NMRI |
| 2 | 32 | 0, 60 | 1.5 µg HA in whole intact inactivated H1N1 | N3 | NMRI |
| 3 | 16 | 0, 60 | 1.5 µg HA in whole intact inactivated H1N1 | pFliC(-gly) | NMRI |
| 4 | 32 | 0, 60 | 1.5 µg HA in whole intact inactivated H1N1 | N3 + pFliC(-gly) | NMRI |
| 5 | 30 | 0, 60 | Saline | N3 + pFliC(-gly) | NMRI |
| 6 | 32 | 0, 56 | 1.5 µg HA in whole intact inactivated H1N1 | None | C57BL/6 |
| 7 | 32 | 0, 56 | 1.5 µg HA in whole intact inactivated H1N1 | N3 | C57BL/6 |
| 8 | 12 | 0, 56 | 1.5 µg HA in whole intact inactivated H1N1 | pFliC(-gly) | C57BL/6 |
| 9 | 32 | 0, 56 | 1.5 µg HA in whole intact inactivated H1N1 | N3 + pFliC(-gly) | C57BL/6 |
| 10 | 24 | 0, 56 | Saline | N3 + pFliC(-gly) | C57BL/6 |
Abbreviations: HA = Hemagglutinine protein from influenza A, pFliC(-gly) = Plasmid encoding secreted Flagellin type C of Salmonella typhimurium with mammalian glycosylation signal sequences removed.