Figure 3.

Phosphorylation reactions of selected pyrimidine nucleosides catalyzed by Py-NPases were monitored via spectral unmixing. (a) Scheme of the general reaction. (b–e) Examples with R = H, Me, F or Ethynyl. All reactions were performed with 2 mM nucleoside, 10 mM K₂HPO₄, 16 µg/mL Py-NPase in 50 mM glycine buffer at pH 9 and 60 °C. Lines between the datapoints were inserted as a guide to the eye.