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Comparison of HPLC and our UV/Vis-based assay for analysis of 2’-deoxythymidine/thymine mixtures (molar fractions of the nucleobase).
| Actual [%] | Predicted [%] | Difference [%] (UV/Vis-Assay - HPLC) |
|
|---|---|---|---|
| HPLC | UV/Vis Assay | ||
| 0 | 0 ± 0 | 1.47 ± 1.02 | +1.47 |
| 20 | 21.08 ± 0.02 | 20.47 ± 0.25 | −0.60 |
| 40 | 40.89 ± 0.15 | 39.46 ± 0.09 | −1.43 |
| 60 | 60.03 ± 0.05 | 58.99 ± 0.38 | −1.04 |
| 80 | 79.38 ± 0.19 | 78.91 ± 0.13 | −0.46 |
| 100 | 99.67 ± 0.36 | 99.85 ± 0.15 | +0.18 |
