Table 1.

Summary of adult female PRM feeding rates and vaccine efficacy.

Group	Hen	Fed Mites	Partially Fed/Unfed Mites	Total	% Fed Mites	Average Feeding ± SD	Reduction	Eggs	Oviposition	Average Oviposition ± SD	Reduction	Dead Mites	Mortality	Average Mortality	Mortality Effect
Deg-AKR	1	56	38/276 = 314	370	15	21 ± 5	0%	34	0.6	0.4 ± 0.2	42% *	4	0.1	0.2 ± 0.1	6%
	2	77	5/343 = 348	425	18			23	0.3			19	0.2
	3	130	23/325 = 348	478	27			34	0.3			16	0.1
	4	120	10/391 = 401	521	23			34	0.3			24	0.2
	5	81	30/246 = 276	357	23			42	0.5			19	0.2
Control	6	100	23/230 = 253	353	28	18 ± 8		65	0.6	0.7 ± 0.2		15	0.2	0.2 ± 0.0
	7	32	32/304 = 336	368	9			23	0.7			4	0.1
	8	59	23/394 = 417	476	12			38	0.6			10	0.2
	9	85	23/284 = 307	392	22			86	1.0			13	0.2
	10	44	34/190 = 224	268	16			15	0.3			10	0.2

Data shown are a compilation from the four assays. Feeding rates were scored on day 1 of the assay, immediately following removal of the feeding devices from the hens. Oviposition and mortality were scored on day 7. Data were analyzed statistically to compare results between mites fed on vaccinated and control hens by GLMM (F = 6.06, p = 0.014, gL1 = 1, gL2 = 747). Fed mites = total of fully engorged adult female mites recovered. Unfed mites = total unfed adult female mites were counted after 3 h placed on the hen. Fed reduction = 1–(Average Feeding Deg-AKR/Average Feeding Control)] × 100. Eggs = total accumulative count of eggs laid at day 7. Oviposition = eggs laid/number fed adult female mites. Oviposition reduction = [1–(Average Oviposition Deg-AKR/Average Oviposition Control)] × 100. GLMM: F = 6.060, p = 0.014. Dead mites = total number of fed adult female mites which shows no response to stimuli and looks dehydrated at day 7. Mortality = dead mites/number fed adult female mites. Mortality effect = [1–(Average Mortality Deg-AKR/Average Mortality Control)] × 100.